Absolutely as long as you aren't fishing clear water. I use fluorocarbon and catch 50:1 over my buddy who uses sufix elite yellow when fishing clear water (15' visibility). He hasn't caught on yet! When the water is dirty the hi-viz line works great.. ESPECIALLY when they are hitting you on the initial drop after a cast.

In the clear water, it really is quite funny to watch the fish swim full steam towards his minnow/jig then stop about a foot away and do a quick 180 and run like a bat out of Hell. Heck they even do it with "clear" mono sometimes.. although its nothing like the colored line. With the fluorocarbon they don't even slow down and inhale the bait.